FLUORIDE STIMULATES [H-3] THYMIDINE INCORPORATION AND ALKALINE-PHOSPHATASE PRODUCTION BY HUMAN OSTEOBLASTS

被引：50

作者：

KHOKHER, MA ^{[1
]}

DANDONA, P ^{[1
]}

机构：

[1] ROYAL FREE HOSP,SCH MED,DEPT CHEM PATHOL & HUMAN METAB,METAB UNIT,POND ST,LONDON NW3 2QG,ENGLAND

来源：

METABOLISM-CLINICAL AND EXPERIMENTAL | 1990年 / 39卷 / 11期

关键词：

D O I：

10.1016/0026-0495(90)90081-M

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The effect of sodium fluoride on alkaline phosphatase (ALP) release and [3H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [3H]thymidine uptake at concentrations of sodium fluoride greater than 250 μmol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis. © 1990.

引用

页码：1118 / 1121

页数：4

共 12 条

[1]

AVASTHY N, 1988, DIABETES RES CLIN EX, V9, P91

[2] PRODUCTION OF OSTEOCALCIN BY HUMAN-BONE CELLS-INVITRO - EFFECTS OF 1,25(OH)2D3, 24,25(OH)2D3, PARATHYROID-HORMONE, AND GLUCOCORTICOIDS [J].

BERESFORD, JN ;

GALLAGHER, JA ;

POSER, JW ;

RUSSELL, RGG .

METABOLIC BONE DISEASE & RELATED RESEARCH, 1984, 5 (05) :229-234

[3]

BRIANCON D, 1981, ORTHOP CLIN N AM, V12, P629

[4] SODIUM-FLUORIDE STIMULATES OSTEOCALCIN IN NORMAL SUBJECTS [J].

DANDONA, P ;

COUMAR, A ;

GILL, DS ;

BELL, J ;

THOMAS, M .

CLINICAL ENDOCRINOLOGY, 1988, 29 (04) :437-441

[5]

FARLEY JR, 1983, SCIENCE, V222, P330

[6] FLUORIDE THERAPY FOR OSTEOPOROSIS - CHARACTERIZATION OF THE SKELETAL RESPONSE BY SERIAL MEASUREMENTS OF SERUM ALKALINE-PHOSPHATASE ACTIVITY [J].

FARLEY, SMG ;

WERGEDAL, JE ;

SMITH, LC ;

LUNDY, MW ;

FARLEY, JR ;

BAYLINK, DJ .

METABOLISM-CLINICAL AND EXPERIMENTAL, 1987, 36 (03) :211-218

[7] THE EFFECT OF NIFEDIPINE, NIMODIPINE AND NISOLDIPINE ON AGONIST-STIMULATED AND TRAUMA-STIMULATED VASCULAR PROSTACYCLIN SYNTHESIS INVITRO [J].

JEREMY, JY ;

MIKHAILIDIS, DP ;

DANDONA, P .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1986, 332 (01) :70-73

[8] EFFECT OF COMBINED THERAPY WITH SODIUM-FLUORIDE VITAMIN-D AND CALCIUM IN OSTEOPOROSIS [J].

JOWSEY, J ;

RIGGS, BL ;

KELLY, PJ ;

HOFFMAN, DL .

AMERICAN JOURNAL OF MEDICINE, 1972, 53 (NJUL) :43-&

[9]

LAU KHW, 1987, J BONE MINER RES S, V2, pA232

[10]

RIGGS BL, 1983, NEW ENGL J MED, V306, P211

← 1 2 →