EXPRESSION OF ERYTHROID-DIFFERENTIATION FACTOR (EDF) IN CHINESE-HAMSTER OVARY CELLS

被引：55
|
作者：
MURATA, M [1 ]
ONOMICHI, K [1 ]
ETO, Y [1 ]
SHIBAI, H [1 ]
MURAMATSU, M [1 ]
机构：
[1] UNIV TOKYO, FAC MED, DEPT BIOCHEM, BUNKYO KU, TOKYO 113, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1988年 / 151卷 / 01期
关键词：
D O I：
10.1016/0006-291X(88)90583-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Plasmid DNA containing EDF subunit cDNA and mouse dihydrofolate reductase (DHFR) cDNA was transfected into CHO DHFR- cells by the calcium-phosphate method. DHFR positive transformants secreted recombinant EDF (r-EDF) constitutively in an active form and accumulated it in the conditioned medium. Furthermore, cells which were resistant to methotrexate (MTX: 0.5 .mu.M) secreted r-EDF up to 1 .mu.g/ml. r-EDF was identical to natural EDF (n-EDF) produced by human acute monocytic leukemia cell line, THP-1, as regards its dimeric structure and a biological activity.
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页码：230 / 235
页数：6
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