An improved method for the detection and quantification of recombinant protein in transgenic plants

被引：2
|
作者：
Longstaff, M
Edmonds, HS
Newell, CA
机构：
[1] AXIS GENET LTD, CAMBRIDGE CB2 4AZ, ENGLAND
[2] UNIV DURHAM, DEPT BIOL SCI, DURHAM DH1 3LE, ENGLAND
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 1995年 / 13卷 / 04期
关键词：
antibody; transgenic plants; recombinant protein; enhanced chemiluminescence;
D O I：
10.1007/BF02669192
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive method has been developed for the detection of recombinant protein produced as a result of gene transfer into plants. This method is based upon antibody binding, which is then visualized using enhanced chemiluminescence and recorded on x-ray film for long-term storage. The technique is simple, rapid and reliable and can be used to screen large numbers of transgenic plants. Several plant species have been successfully tested in this way for a range of recombinant proteins.
引用
收藏
页码：363 / 368
页数：6
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