TRANSFORMING GROWTH-FACTOR-BETA-2 IS AN AUTOCRINE GROWTH INHIBITORY FACTOR FOR THE MOSER HUMAN COLON-CARCINOMA CELL-LINE

被引：15
|
作者：
LEVINE, AE [1 ]
LEWIS, LR [1 ]
机构：
[1] UNIV TEXAS, HLTH SCI CTR, DEPT BIOL CHEM, HOUSTON, TX 77225 USA
来源：
CANCER LETTERS | 1993年 / 68卷 / 01期
关键词：
TRANSFORMING GROWTH FACTOR-BETA; AUTOCRINE; COLON CARCINOMA; DIFFERENTIATION; GROWTH INHIBITION;
D O I：
10.1016/0304-3835(93)90216-V
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The MOSER human colon carcinoma cell line is significantly growth inhibited by exogenous transforming growth factor-beta (TGF-beta). The secretion of TGF-beta by these cells was examined to determine if endogenous TGF-beta might also regulate MOSER cell growth. MOSER cells secreted 11 ng TGF-beta/10(6) cells, 24 % of which was in the active form. Blocking antibodies specific for TGF-beta2 stimulated growth 1.4-fold, while TGF-beta1 specific antibodies were without effect. Treatment of MOSER cells with the differentiation agent, N,N-dimethylformamide (DMF), inhibited cell growth and resulted in an 8-fold increase in secreted TGF-beta (20 % active). Only antibodies specific for TGF-beta2 were able to reverse the growth inhibitory effect of DMF on these cells. Therefore, TGF-beta2 acted as a negative autocrine inhibitory factor for MOSER cells and the growth inhibitory effects of DMF were mediated by the increased secretion of active TGF-beta2.
引用
收藏
页码：33 / 41
页数：9
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