THE PYRUVATE KINASE-COUPLED ASSAY FOR ATPASES - A CRITICAL ANALYSIS

被引：26
作者：
JENKINS, WT
机构：
[1] Department of Chemistry, Indiana University, Bloomington
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 01期
关键词：
D O I：
10.1016/0003-2697(91)90160-U
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The pyruvate kinase assay for ATPases suffers from major systematic errors due to the variable amounts of the inhibitory product, ADP, present as the rates change. A simple method is presented to estimate the true uninhibited rates and the ADP inhibition constant for the ATPase. © 1991.
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收藏
页码：136 / 139
页数：4
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