PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST A HUMAN OVARIAN TERATOCARCINOMA CELL-LINE

被引：0
作者：
LEMIEUX, P [1 ]
PAGE, M [1 ]
机构：
[1] UNIV LAVAL,FAC MED,DEPT BIOCHEM,ST FOY,PQ G1K 7P4,CANADA
来源：
ANTICANCER RESEARCH | 1994年 / 14卷 / 6B期
关键词：
MONOCLONAL ANTIBODIES; OVARIAN CANCER; MUCIN; 4E3; ANTIGEN;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Nine monoclonal antibodies (MAbs) of a total of 25 clones selected by an enzyme immunoassy (ELISA) were produced against a human ovarian teratocarcinoma cell line. Within the panel, one antibody (4E3) has shown a close reactivity to OC-125. However, the antigen recognized by 4E3 was found to be different from the CA-125 antigen, The cell surface antigen recognized by 4E3 has a molecular weight over 2 x 10(6) Da and is highly glycosylated as shown by the periodic acid/Schiff reagent.
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收藏
页码：2709 / 2715
页数：7
相关论文
共 27 条
[1]   TRANS-VAGINAL COLOR FLOW IMAGING - A POSSIBLE NEW SCREENING TECHNIQUE FOR OVARIAN-CANCER [J].
BOURNE, T ;
CAMPBELL, S ;
STEER, C ;
WHITEHEAD, MI ;
COLLINS, WP .
BRITISH MEDICAL JOURNAL, 1989, 299 (6712) :1367-1370
[2]   HUMAN OVARIAN CANCERS SPECIFICALLY BIND DAUNORUBICIN-OC-125 CONJUGATE - AN IMMUNOFLUORESCENCE STUDY [J].
DEZSO, B ;
TOROK, I ;
ROSIK, LO ;
SWEET, F .
GYNECOLOGIC ONCOLOGY, 1990, 39 (01) :60-64
[3]   ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].
FAIRBANKS, G ;
STECK, TL ;
WALLACH, DFH .
BIOCHEMISTRY, 1971, 10 (13) :2606-+
[4]  
FRAKER PJ, 1978, BIOCHEM BIOPH RES CO, V80, P849, DOI 10.1016/0006-291X(78)91322-0
[5]  
JACOBS I, 1989, HUM REPROD, V4, P1
[6]  
JOHNSON VG, 1986, CANCER RES, V46, P850
[7]  
KAWATA M, 1991, CANCER RES, V51, P2655
[8]   CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].
KOHLER, G ;
MILSTEIN, C .
NATURE, 1975, 256 (5517) :495-497
[9]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[10]   THE EFFECT OF RETINOIC ACID ON CHEMOSENSITIVITY OF PA-1 HUMAN TERATOCARCINOMA CELLS AND ITS MODULATION BY AN ACTIVATED N-RAS ONCOGENE [J].
LERUPPERT, K ;
MASTERS, JRW ;
KNUECHEL, R ;
SEEGERS, S ;
TAINSKY, MA ;
HOFSTAEDTER, F ;
BUETTNER, R .
INTERNATIONAL JOURNAL OF CANCER, 1992, 51 (04) :646-651
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