CHROMATIN ASSEMBLY ON REPLICATING DNA INVITRO

被引：91
作者：
ALMOUZNI, G
CLARK, DJ
MECHALI, M
WOLFFE, AP
机构：
[1] NIDDK, MOLEC BIOL LAB, BETHESDA, MD 20892 USA
[2] UNIV PARIS 07, CNRS, INST JACQUES MONOD, F-75221 PARIS 05, FRANCE
来源：
NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 19期
关键词：
D O I：
10.1093/nar/18.19.5767
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Replicating single-stranded DNA is preferentially assembled Into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histories H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract. © 1990 Oxford University Press.
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页码：5767 / 5774
页数：8
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