Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing

被引：42

作者：

Jin, Hongjun ^{[1
]}

Yu, Yuehua ^{[1
]}

Chrisler, William B. ^{[1
]}

Xiong, Yijia ^{[1
]}

Hu, Dehong ^{[2
]}

Lei, Chenghong ^{[1
]}

机构：

[1] Pacific Northwest Natl Lab, Cell Biol & Biochem Grp, Richland, WA 99352 USA

[2] Pacific Northwest Natl Lab, Fundamental & Computat Sci Directorate, Chem Phys & Anal Grp, Richland, WA 99352 USA

来源：

BREAST CANCER-BASIC AND CLINICAL RESEARCH | 2012年 / 6卷

关键词：

microRNA-10b; breast cancer metastasis; nanoparticles;

D O I：

10.4137/BCBCR.S8513

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.

引用

页码：9 / 19

页数：11

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