HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR THE SIMULTANEOUS DETERMINATION OF IPRATROPIUM BROMIDE, FENOTEROL, SALBUTAMOL AND TERBUTALINE IN NEBULIZER SOLUTION

A reversed-phase ion-pair high-performance liquid chromatography assay was developed for the simultaneous determination of ipratropium bromide, fenoterol hydrobromide, salbutamol sulphate and terbutaline sulphate in nebulizer solution. Chromatographic separation was achieved with a Nova-Pak C-18 4 mu m 10 cm x 8 mm i.d. Radial-pak cartridge inside a Waters RCM 8 x 10 compression module using ternary gradient analysis. Detection was performed using UV detection at 220 nm. The standard curves were linear over the following ranges: ipratropium bromide 20.8-250.0 mu g ml(-1), fenoterol hydrobromide 27.8-500.0 mu g 8 ml(-1), salbutamol sulphate 34.7-2500.0 mu g ml(-1) and terbutaline sulphate 69.5-2500 mu g ml(-1). Inter-day and intra-day relative standard deviations for each compound ranged from 4.5-5.2% and 3.5-3.9%, respectively. The assay procedure was developed to allow the accurate determination of constituents in various combinations of nebulizer solution, as well as for stability indicating purposes. This provides a convenient means of testing long-term compatibility and stability following the post-manufacture mixing of commonly used nebulized preparations.