SPECIFICITY OF THE ISOLECTINS FROM THE PLANT CACTUS MACHAEROCEREUS-ERUCA FOR OLIGOSACCHARIDES FROM PORCINE STOMACH MUCIN

被引：18
作者：
ZENTENO, E
VAZQUEZ, L
CHAVEZ, R
CORDOBA, F
WIERUSZESKI, JM
MONTREUIL, J
DEBRAY, H
机构：
[1] INST NACL ENFERMEDADES RESP, DEPT BIOQUIM, TLALPAN 14080, DF, MEXICO
[2] UNIV AUTONOMA ESTADO MORELOS, CTR INVEST BIOL J FELIX FRIAS, BIOL EXPTL LAB, CUERNAVACA 68290, MORELOS, MEXICO
[3] Univ Nacl Autonoma Mexico, FAC MED, DEPT BIOQUIM, MEXICO CITY 04510, DF, MEXICO
[4] UNIV SCI & TECH LILLE FLANDRES ARTOIS, CHIM BIOL LAB, F-59655 VILLENEUVE DASCQ, FRANCE
来源：
GLYCOCONJUGATE JOURNAL | 1995年 / 12卷 / 05期
关键词：
CACTUS LECTINS; MUCIN SPECIFIC LECTINS; GLYCOPROTEINS; PLANT LECTINS;
D O I：
10.1007/BF00731267
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Sugar specificity of the Machaerocereus eruca isolectins, MeA(I) and MeA(II) has been determined by comparing the capacity of glycans with well defined structures to inhibit their haemagglutinating activity. Both are galactose-specific isolectins with high affinity for O-glycans. However, the two M. eruca isolectins recognize different oligosaccharidic sequences belonging to O-glycosidically linked glycans from porcine stomach mucin. The minimal structure recognized by MeA, on the porcine mucin glycans is the O-glycan core Gal beta 1,3GalNAc-ol, whereas MeA(II) has a more extended site and interacts with a biantennary O-glycan possessing the terminal trisaccharide Fuc alpha 1,2 (GalNAc alpha 1,3) Gal beta 1,4.
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页码：699 / 706
页数：8
相关论文
共 27 条
[1]  
BAILLY P, 1985, GLYCOCONJUGATE J, V2, P410
[2]  
CARLSEN RB, 1991, J BIOL CHEM, V242, P5474
[3]   LECTINS, LECTIN GENES, AND THEIR ROLE IN PLANT DEFENSE [J].
CHRISPEELS, MJ ;
RAIKHEL, NV .
PLANT CELL, 1991, 3 (01) :1-9
[4]   BINDING OF HUMAN FIBROBLAST INTERFERON TO CONCANAVALIN A-AGAROSE - INVOLVEMENT OF CARBOHYDRATE RECOGNITION AND HYDROPHOBIC INTERACTION [J].
DAVEY, MW ;
SULKOWSKI, E ;
CARTER, WA .
BIOCHEMISTRY, 1976, 15 (03) :704-713
[5]  
DUA VK, 1986, J BIOL CHEM, V261, P1599
[6]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[7]   EFFECT OF PH ON THE BINDING OF VICIA-GRAMINEA LECTIN TO ERYTHROCYTES - DEPENDENCE ON THE CHEMICAL CHARACTER OF RED-CELL RECEPTORS [J].
DUK, M ;
LISOWSKA, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 143 (01) :73-78
[8]  
Goldstein, 1986, LECTINS PROPERTIES F
[9]   PURIFICATION AND CHARACTERIZATION OF A GALACTOSE-SPECIFIC LECTIN FROM PSILOCYBE-BARRERAE [J].
HERNANDEZ, E ;
ORTIZ, R ;
LOPEZ, F ;
MASSO, F ;
MONTANO, LF ;
MARTINAGE, A ;
ZENTENO, E .
PHYTOCHEMISTRY, 1993, 32 (05) :1209-1211
[10]  
IYER RN, 1971, ARCH BIOCHEM BIOPHYS, V142, P301
123