G-CSF - AN ANTIINFLAMMATORY CYTOKINE

被引:18
|
作者
HARTUNG, T
VOLK, HD
WENDEL, A
机构
[1] UNIV KONSTANZ,DEPT BIOCHEM PHARMACOL,D-78434 CONSTANCE,GERMANY
[2] CHARITE BERLIN,DEPT CLIN IMMUNOL,BERLIN,GERMANY
来源
JOURNAL OF ENDOTOXIN RESEARCH | 1995年 / 2卷 / 03期
关键词
D O I
10.1177/096805199500200308
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have previously reported that in various macrophage populations prepared from G-CSF treated rats LPS-inducible TNF release was suppressed, In vitro, LPS induced liver cell death only when hepatocytes were cocultured with liver macrophages, Rat Kupffer cells from G-CSF treated donor animals were less potent in mediating LPS-inducible hepatocytotoxicity in vitro than cells from control animals, These ex vivo findings were confirmed in vivo by demonstrating that G-CSF treatment attenuated LPS-inducible circulating TNF levels and protected from liver injury and mortality, We extended these observations to humans in two studies with G-CSF treated volunteers, In a pilot study, 11 subjects were treated single-blindly with 480 mu g G-CSF s.c. (n = 7) or saline placebo (n = 4), Blood was taken at different time-points relative to G-CSF injection and cytokine release capacity was assessed in LPS stimulated whole blood incubations, In blood from G-CSF treated volunteers, we found reduced LPS-inducible TNF formation while the release of both soluble TNF receptor (sTNF-R) and interleukin 1 receptor antagonist (IL-1ra) were increased. In a second double-blind, randomized and controlled study, three groups of seven volunteers were treated once or twice 24 h apart with G-CSF or solvent placebo, Besides LPS, various stimuli were included to initiate cytokine release in a whole blood assay, The reduction of TNF formation (mean 53% at 24 h after G-CSF) was different with the various stimuli, All stimuli increased IL-1ra (mean 14-fold) and sTNF-R (mean 3-fold) at 24 h after G-CSF, LPS-inducible IFN-gamma and GM-CSF were significantly reduced, Our data indicate that the pattern of cytokines produced by human whole blood taken after G-CSF treatment in response to a variety of stimuli is shifted from pro- to anti-inflammatory mediators, These findings extend the knowledge on the pharmacology of G-CSF in animal models of the systemic inflammatory response syndrome and prompt a trial of G-CSF prophylaxis with this indication.
引用
收藏
页码:195 / 201
页数:7
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