DETERMINATION OF H(N),H-ALPHA AND H(N), C' COUPLING-CONSTANTS IN C-13,N-15-LABELED PROTEINS

被引：0

作者：

WEISEMANN, R

RUTERJANS, H

SCHWALBE, H

SCHLEUCHER, J

BERMEL, W

GRIESINGER, C

机构：

[1] UNIV FRANKFURT,INST ORGAN CHEM,MARIE CURIE STR 11,D-60439 FRANKFURT,GERMANY

[2] UNIV FRANKFURT,INST BIOPHYS CHEM,D-60439 FRANKFURT,GERMANY

[3] BRUKER ANALYT MESSTECH GMBH,D-76287 RHEINSTETTEN,GERMANY

来源：

JOURNAL OF BIOMOLECULAR NMR | 1994年 / 4卷 / 02期

关键词：

3D NMR; J-COUPLING CONSTANTS; ISOTOPIC LABELING; PROTEIN STRUCTURE; PHI-ANGLE; SOFT HNCA-COSY; SOFT HNCA-E-COSY; GRADIENT-ENHANCED SPECTROSCOPY; SENSITIVITY-ENHANCED SPECTROSCOPY; RIBONUCLEASE-T1;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(H(N),H(alpha) and 3J(H(N),C') coupling constants in uniformly C-13- and N-15-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the phi-angle for protein structure determination. The method is applied to uniformly C-13,N-15-labeled ribonuclease T1.

引用

页码：231 / 240

页数：10

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