USE OF ELECTROSPRAY MASS-SPECTROMETRY TO INVESTIGATE THE INHIBITION OF BETA-LACTAMASES BY 6-HALOGENOPENICILLANIC ACIDS

被引：12
作者：
APLIN, RT
ROBINSON, CV
SCHOFIELD, CJ
WALEY, SG
机构：
[1] LAB MOLEC BIOPHYS,OXFORD OX1 3QY,ENGLAND
[2] OXFORD CTR MOLEC SCI,OXFORD OX1 3QY,ENGLAND
来源：
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS | 1993年 / 02期
关键词：
D O I：
10.1039/c39930000121
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Electrospray mass spectrometry has been used to investigate the mechanism of inhibition of class A and C beta-lactamases by 6-halogenopenicillanic acids; in the case of the 6beta-halogen substituted penicillanic acids the molecular mass differences observed between the unreacted and the inhibited beta-lactamases are consistent with the formation of an enzyme bound dihydrothiazine derivative, as previously proposed.
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页码：121 / 123
页数：3
相关论文
共 13 条
[1]   USE OF ELECTROSPRAY MASS-SPECTROMETRY TO DIRECTLY OBSERVE AN ACYL ENZYME INTERMEDIATE IN BETA-LACTAMASE CATALYSIS [J].
APLIN, RT ;
BALDWIN, JE ;
SCHOFIELD, CJ ;
WALEY, SG .
FEBS LETTERS, 1990, 277 (1-2) :212-214
[2]   INACTIVATION OF BACILLUS-CEREUS BETA-LACTAMASE-I BY 6-BETA-BROMOPENICILLANIC ACID - MECHANISM [J].
COHEN, SA ;
PRATT, RF .
BIOCHEMISTRY, 1980, 19 (17) :3996-4003
[3]   SEPARATION, PURIFICATION AND PROPERTIES OF BETA-LACTAMASE I AND BETA-LACTAMASE II FROM BACILLUS-CEREUS 569-H-9 [J].
DAVIES, RB ;
ABRAHAM, EP ;
MELLING, J .
BIOCHEMICAL JOURNAL, 1974, 143 (01) :115-127
[4]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[5]  
JORIS B, 1988, BIOCHEM J, V187, P797
[6]   6-BETA-BROMOPENICILLANIC ACID INACTIVATES BETA-LACTAMASE-I [J].
KNOTTHUNZIKER, V ;
ORLEK, BS ;
SAMMES, PG ;
WALEY, SG .
BIOCHEMICAL JOURNAL, 1979, 177 (01) :365-367
[7]   INACTIVATION OF BACILLUS-CEREUS BETA-LACTAMASE-I BY 6-BETA-BROMOPENICILLANIC ACID - KINETICS [J].
LOOSEMORE, MJ ;
COHEN, SA ;
PRATT, RF .
BIOCHEMISTRY, 1980, 19 (17) :3990-3995
[8]   ON THE CHEMISTRY OF BETA-LACTAMASE INHIBITION BY 6-BETA-BROMOPENICILLANIC ACID [J].
ORLEK, BS ;
SAMMES, PG ;
KNOTTHUNZIKER, V ;
WALEY, SG .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1980, (10) :2322-2329
[9]   6-BETA-BROMOPENICILLANIC ACID, A POTENT BETA-LACTAMASE INHIBITOR [J].
PRATT, RF ;
LOOSEMORE, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (09) :4145-4149
[10]   NEW DEVELOPMENTS IN BIOCHEMICAL MASS-SPECTROMETRY - ELECTROSPRAY IONIZATION [J].
SMITH, RD ;
LOO, JA ;
EDMONDS, CG ;
BARINAGA, CJ ;
UDSETH, HR .
ANALYTICAL CHEMISTRY, 1990, 62 (09) :882-899
12