IDENTIFICATION OF BROCCOLI AND CAULIFLOWER CULTIVARS WITH RAPD MARKERS

被引：88

作者：

HU, J ^{[1
]}

QUIROS, CF ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT VEGETABLE CROPS,DAVIS,CA 95616

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 10期

关键词：

BRASSICA-OLERACEA; GENETIC MARKERS; VARIETAL IDENTIFICATION; DNA POLYMORPHISM;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

RAPD (Random Amplified Polymorphic DNA) markers generated by 4 arbitrary 10-mer primers, discriminated 14 broccoli and 12 cauliflower cultivars (Brassica oleracea L.) by banding profiles. The size of the amplified DNA fragments ranged from 300 to 2600 base pairs. Twenty-eight percent of the markers were fixed in both broccoli and cauliflower, whereas 12.5% were specific to either crop. The rest were polymorphic in either or both crops. The markers generated by two and three primers were sufficient to distinguish each of the broccoli and cauliflower cultivars, respectively. The average difference in markers was 14.5 between broccoli and cauliflower markers, 5.8 between two broccoli cultivars and 7.9 between two cauliflower cultivars. Larger differences for each crop were found between cultivars from different seed companies than within the same company. RAPD markers provide a quick and reliable alternative to identify broccoli and cauliflower cultivars.

引用

页码：505 / 511

页数：7

共 16 条

[1] ELECTROPHORETIC VARIATION AS A TOOL FOR DETERMINING SEED PURITY AND FOR BREEDING HYBRID VARIETIES OF BRASSICA-OLERACEA [J].