CHARACTERIZATION OF A NEW STRAIN OF TOBACCO NECROSIS VIRUS ISOLATED FROM NUTRIENT FEEDING SOLUTION

被引：2

作者：

ADAM, G ^{[1
]}

WINTER, S ^{[1
]}

LESEMANN, DE ^{[1
]}

机构：

[1] BIOL BUNDESANSTALT LAND & FORSTWIRTSCHAFT, BRAUNSCHWEIG, GERMANY

来源：

ANNALS OF APPLIED BIOLOGY | 1990年 / 116卷 / 03期

关键词：

cDNA‐clones; cytopathology; electron microscopy; immunoelectron microscopy; isolation and purification; serology;

D O I：

10.1111/j.1744-7348.1990.tb06635.x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A virus, isolated from the nutrient feeding solution of a bell pepper culture in a glasshouse, was characterised as a new serotype of tobacco necrosis necrovirus (TNV) and named TNV‐nft. The virus was monopartite and contained a single RNA species and a single coat protein with molecular weights of 1.45± 106 and 2.9 ± 104 daltons, respectively. The particle had an apparent sedimentation coefficient of 110 S and a density of 1.37 g/cm3 in cesium chloride. In decoration tests antiserum prepared against TNV‐nft reacted most strongly with the TNV strains Kassanis A and B, but at lower dilutions it also reacted with all other TNV‐serotypes available. In Ouchterlony tests spur formation occurred with TNV strains Kassanis A and B, indicating that the TNV‐nft strain was serologically distinct. The isolate was not associated with a satellite virus. Cloned cDNA prepared against viral RNA reacted exclusively with the viral RNA band in Northern blots. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：523 / 536

页数：14

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