CLONING AND EXPRESSION OF A GENE ENCODING AN INTERLEUKIN-3 RECEPTOR-LIKE PROTEIN - IDENTIFICATION OF ANOTHER MEMBER OF THE CYTOKINE RECEPTOR GENE FAMILY

被引:190
作者
GORMAN, DM
ITOH, N
KITAMURA, T
SCHREURS, J
YONEHARA, S
YAHARA, I
ARAI, KI
MIYAJIMA, A
机构
[1] DNAX RES INST MOLEC & CELLULAR BIOL INC,MOLEC & CELLULAR BIOL RES INST,901 CALIF AVE,PALO ALTO,CA 94304
[2] UNIV TOKYO,INST MED SCI,MINATO KU,TOKYO 108,JAPAN
[3] TOKYO METROPOLITAN INST MED SCI,BUNKYO KU,TOKYO 113,JAPAN
关键词
Colony-stimulating factor; Hemopoietic growth factor; Lymphokine; Lymphokine receptor;
D O I
10.1073/pnas.87.14.5459
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Using a monoclonal antibody to the interleukin 3 (IL-3) receptor (anti-Aic2), we isolated a cDNA (AIC2B) from a mouse mast cell line which is homologous to the previously characterized gene for the IL-3 receptor (AIC2A). This cDNA encodes a polypeptide of 896 amino acid residues and has 91% amino acid sequence identity with the IL-3 receptor. A consensus sequence defining an additional cytokine receptor family is present in this clone. Compared to the AIC2A clone, the AIC2B cDNA encodes a protein with amino acid substitutions, insertions, and deletions dispersed throughout the entire protein. Oligonucleotide probes specific for each cDNA hybridized with different genomic fragments, indicating that the AIC2A and AIC2B proteins are encoded by two distinct genes. Fibroblasts transfected with the AIC2B cDNA expressed the protein at the cell surface as determined by binding with the anti-Aic2 antibody but did not bind IL-3 or other cytokines, including Il-2, IL-4, granulocyte-macrophage colony-stimulating factor, erythropoietin, and Il-9 (p40) at concentrations between 1 and 10 nM. An S1 nuclease protection assay was used to discriminate between the AIC2A and AIC2B transcripts. We found that the AICB2 gene was coexpressed with the AIC2A gene. These results suggest a potential involvement of AIC2B in cytokine signal transduction.
引用
收藏
页码:5459 / 5463
页数:5
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