EXPRESSION OF BOTH BACILLUS-SUBTILIS THREONYL-TRANSFER-RNA SYNTHETASE GENES IS AUTOGENOUSLY REGULATED

被引:33
作者
GENDRON, N [1 ]
PUTZER, H [1 ]
GRUNBERGMANAGO, M [1 ]
机构
[1] INST BIOL PHYSICOCHIM,CNRS,URA 1139,SERV BIOCHIM,13 RUE PIERRE & MARIE CURIE,F-75005 PARIS,FRANCE
关键词
D O I
10.1128/JB.176.2.486-494.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ''housekeeping'' threonyl-tRNA synthetase gene (thrS) of Bacillus subtilis is shown to be transcribed in vivo and in vitro from a single promoter. In vitro, 85% of all messages transcribed from the thrS promoter are terminated at a strong factor-independent terminator localized upstream of the thrS Shine-Dalgarno sequence, within the 305-nucleotide-long leader region. Overexpression of thrS represses transcriptional and translational thrS-lacZ fusions to a similar extent, suggesting that thrS is autoregulated at the transcriptional level. We show that autogenous control does not act at the level of transcription initiation but involves antitermination of the transcription mechanism. thrZ, the second threonyl-tRNA synthetase gene, is also autogenously regulated. However, the ability of the ThrS synthetase to repress thrS as well as thrZ expression is much greater than that of the ThrZ synthetase.
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页码:486 / 494
页数:9
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