DEXAMETHASONE REGULATES OBESE EXPRESSION IN ISOLATED RAT ADIPOCYTES

被引:208
作者
MURAKAMI, T
IIDA, M
SHIMA, K
机构
[1] UNIV TOKUSHIMA,SCH MED,DEPT LAB MED,TOKUSHIMA 770,JAPAN
[2] OTSUKA PHARMACEUT CO LTD,DIV DIAGNOST,TOKUSHIMA 77101,JAPAN
关键词
D O I
10.1006/bbrc.1995.2422
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An obese (ob) gene product, expressed specifically in adipose tissues, regulates energy balance. Here we report that adipocytes in adipose tissue actually express ob mRNA and that a synthetic glucocorticoid (dexamethasone) regulates expression of the ob gene. Addition of 100 nM dexamethasone to isolated rat adipocytes rapidly induced a 4-8 fold increase in ob mRNA. This increase in oh mRNA level was apparent within 1 h, and reached a maximum at about 7 h after stimulation. The dexamethasone-stimulated increase of ob mRNA was only partially blocked by protein synthesis inhibitors cycloheximide (20 mu g/ml) or anisomycin (200 mu M). This suggests that new protein synthesis is not necessarily required for the observed dexamethasone-stimulated increase in ob mRNA. (C) 1995 Academic Press, Inc.
引用
收藏
页码:1260 / 1267
页数:8
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