EXTENDED N-TERMINAL SEQUENCING OF PROTEINS OF THE LARGE RIBOSOMAL-SUBUNIT FROM YEAST MITOCHONDRIA

被引：41
|
作者：
GROHMANN, L [1 ]
GRAACK, HR [1 ]
KRUFT, V [1 ]
CHOLI, T [1 ]
GOLDSCHMIDTREISIN, S [1 ]
KITAKAWA, M [1 ]
机构：
[1] MAX PLANCK INST MOLEC GENET,ABT WITTMANN,W-1000 BERLIN 33,GERMANY
来源：
FEBS LETTERS | 1991年 / 284卷 / 01期
关键词：
MITORIBOSOMAL PROTEIN; AMINO ACID SEQUENCING; MITOCHONDRIA; YEAST; SACCHAROMYCES-CEREVISIAE;
D O I：
10.1016/0014-5793(91)80759-V
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have determined the N-termini of 26 proteins of the large ribosomal subunit from yeast mitochondria by direct amino acid micro-sequencing. The N-terminal sequences of proteins YmL33 and YmL38 showed a significant similarity to eubacterial ribosomal (r-) proteins L30 and L14, respectively. In addition, several proteins could be assigned to their corresponding yeast nuclear genes. Based on a comparison of the protein sequences deduced from the corresponding DNA regions with the N-termini of the mature proteins, the putative leader peptides responsible for mitochondrial matrix-targeting were compiled. In most leader sequences a relative abundance of aromatic amino acids, preferentially phenylalanine, was found.
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页码：51 / 56
页数：6
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