EXTENDED N-TERMINAL SEQUENCING OF PROTEINS OF THE LARGE RIBOSOMAL-SUBUNIT FROM YEAST MITOCHONDRIA

被引：41

作者：

GROHMANN, L ^{[1
]}

GRAACK, HR ^{[1
]}

KRUFT, V ^{[1
]}

CHOLI, T ^{[1
]}

GOLDSCHMIDTREISIN, S ^{[1
]}

KITAKAWA, M ^{[1
]}

机构：

[1] MAX PLANCK INST MOLEC GENET,ABT WITTMANN,W-1000 BERLIN 33,GERMANY

来源：

FEBS LETTERS | 1991年 / 284卷 / 01期

关键词：

MITORIBOSOMAL PROTEIN; AMINO ACID SEQUENCING; MITOCHONDRIA; YEAST; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(91)80759-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the N-termini of 26 proteins of the large ribosomal subunit from yeast mitochondria by direct amino acid micro-sequencing. The N-terminal sequences of proteins YmL33 and YmL38 showed a significant similarity to eubacterial ribosomal (r-) proteins L30 and L14, respectively. In addition, several proteins could be assigned to their corresponding yeast nuclear genes. Based on a comparison of the protein sequences deduced from the corresponding DNA regions with the N-termini of the mature proteins, the putative leader peptides responsible for mitochondrial matrix-targeting were compiled. In most leader sequences a relative abundance of aromatic amino acids, preferentially phenylalanine, was found.

引用

页码：51 / 56

页数：6

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