COMPARISON OF AN HPLC AND BIOASSAY METHOD TO DETERMINE ANTIMICROBIAL CONCENTRATIONS AFTER INTRAVENOUS AND ORAL-ADMINISTRATION OF ENROFLOXACIN IN 4 DOGS

被引：36

作者：

KUNG, K

RIOND, JL

WOLFFRAM, S

WANNER, M

机构：

[1] Division of Animal Nutrition, University of Zürich, CH-8057 Zürich

[2] Institute of Veterinary Physiology, University of Zürich, CH-8057 Zürich

[3] Division of Animal Nutrition, Institute of Veterinary Physiology, University of Zürich, CH-8057 Zürich

来源：

RESEARCH IN VETERINARY SCIENCE | 1993年 / 54卷 / 02期

关键词：

D O I：

10.1016/0034-5288(93)90065-N

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Plasma samples of dogs given 5 mg kg-1 bodyweight of a broad spectrum antimicrobial with the active ingredient enrofloxacin were assayed by two different methods (bioassay: Escherichia coli 14 ICB 4004 on iso sensitest agar; high performance liquid chromatography with a RP C18 column). At concentrations up to 1000 ng ml-1 a linear correlation between values obtained by the two assay methods was found. At concentrations above 1000 ng ml-1 no correlation could be determined. The main metabolite of enrofloxacin, ciprofloxacin, is an important determinant of overall antimicrobial activity and hence influences bioassay results.

引用

页码：247 / 248

页数：2

共 9 条

[1] INTRAMUSCULAR AND ORAL DISPOSITION OF ENROFLOXACIN IN AFRICAN GRAY PARROTS FOLLOWING SINGLE AND MULTIPLE DOSES [J].