NITRIC-OXIDE IN BIOLOGICAL-FLUIDS - ANALYSIS OF NITRITE AND NITRATE BY HIGH-PERFORMANCE ION CHROMATOGRAPHY

被引：82

作者：

EVERETT, SA ^{[1
]}

DENNIS, MF ^{[1
]}

TOZER, GM ^{[1
]}

PRISE, VE ^{[1
]}

WARDMAN, P ^{[1
]}

STRATFORD, MRL ^{[1
]}

机构：

[1] MT VERNON HOSP,CANC RES CAMPAIGN,GRAY LAB,NORTHWOOD HA6 2JR,MIDDX,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 706卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00078-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of nitric oxide-derived nitrite and nitrate ions in biological fluids represents a proven strategy for determining nitric oxide participation in a diverse range of physiological and pathophysiological processes in vivo. In this article we describe a versatile method for the simultaneous measurement of NO2- and NO3- anions in both plasma and isolated tumour models based on anion-exchange chromatography with spectrophotometric detection (214 nm). This method compares well with the capillary electrophoresis technique, exhibiting an equivalent sensitivity for NO2-/NO3- anions and short run-times, i.e. not greater than 4 min. Comparisons are also made with two alternative but less satisfactory methods which employ ion-exchange or reversed-phase ion-pair chromatography with conductimetric as well as spectrophotometric detection. Technical problems associated with each method, particularly those arising from nitrate contamination, have been addressed.

引用

页码：437 / 442

页数：6

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