NESTED PCR METHOD FOR RAPID AND SENSITIVE DETECTION OF VIBRIO-VULNIFICUS IN FISH, SEDIMENTS, AND WATER

被引：68
作者：
ARIAS, CR [1 ]
GARAY, E [1 ]
AZNAR, R [1 ]
机构：
[1] UNIV VALENCIA,DEPT MICROBIOL,E-46100 BURJASSOT,SPAIN
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 09期
关键词：
D O I：
10.1128/AEM.61.9.3476-3478.1995
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
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页码：3476 / 3478
页数：3
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