LIGATION OF HLA CLASS-II MOLECULES PROMOTES SENSITIVITY TO CD95 (FAS ANTIGEN, APO-1)-MEDIATED APOPTOSIS

被引:36
作者
YOSHINO, T
CAO, L
NISHIUCHI, R
MATSUO, Y
YAMADORI, I
KONDO, E
TERAMOTO, N
HAYASHI, K
TAKAHASHI, K
KAMIKAWAJI, N
AKAGI, T
机构
[1] HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR,OKAYAMA,JAPAN
[2] KYUSHU UNIV,MED INST BIOREGULAT,FUKUOKA 812,JAPAN
关键词
HLA CLASS II; FAS ANTIGEN; APO-1; CD95; APOPTOSIS;
D O I
10.1002/eji.1830250811
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
CD95 (Fas antigen/APO-1) is up-regulated in activated lymphocytes, and monoclonal antibody (mAb) to CD95 induces apoptosis. HLA class II molecules play a key role in antigen presentation, ligation of which induces signal transduction. We examined 18 lymphoid cell lines (15 B cell and 3 T cell lines) to investigate the effects of ligation of HLA class II molecules on CD95-mediated apoptosis. All of the five immature B cell lines were sensitive to anti-CD95 mAb, and ligation of HLA class II molecules promoted CD95-mediated apoptosis. In seven B-blastoid cell lines, two Burkitt lines were resistant to anti-CD95 mAb in spite of high expression of CD95. In three of five non-Burkitt B-blastoid lines, CD95-mediated apoptosis was augmented by treatment with anti-HLA class II mAb, while the other two lines lacking CD95 were resistant to anti-CD95 mAb. Three plasmacytic cell lines showed CD95-mediated apoptosis, but enhancement by anti-HLA class II mAb was slight in one cell line and was not observed in the other two lines. Out of three HLA class II antigen-positive T cell lines, CD95-mediated apoptosis was observed to some degree in one cell line but was not promoted by the treatment with anti-KLA class II mAb, and the other two cell lines were resistant to anti-CD95 mAb. Ligation of HLA class II molecules did not alter CD95 expression in the five cell lines examined, except Su-DHL-4 originated from a follicular lymphoma, which showed slight up-regulation. Taken together, ligation of HLA class II molecules apparently promotes CD95-mediated apoptosis in immature B cells and non-Burkitt a blasts. These findings highlight the role of HLA class LI molecules in CD95-mediated apoptosis, which may facilitate rapid clearance of functionally useless cells from the: immune system and might be involved in negative selection of B cells.
引用
收藏
页码:2190 / 2194
页数:5
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