PARAMETERS AFFECTING THE ADSORPTION OF LIGANDS TO POLYVINYL-CHLORIDE PLATES IN ENZYME IMMUNOASSAYS

被引:4
作者
ZALAZAR, FE
CHIABRANDO, GA
ALDAO, MAJ
VIDES, MA
机构
[1] Departamento de Bioquimica Clínica, Facultad de Ciencias Quimicas, Universidad Nacional de Córdoba, 5016 Córdoba
关键词
POLYVINYL CHLORIDE; IMMUNOASSAY; LIGAND ADSORPTION; KINETICS OF ADSORPTION;
D O I
10.1016/0022-1759(92)90082-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In the present work, we studied the efficacy of three blocking agents (HSA, BSA and OVA) in the inhibition of non-specific binding to PVC plates. According to the inhibition data, 1% OVA was the most effective blocking agent. On the other hand, the presence of detergents in all of the blocking solutions drastically decreased the percent inhibition of the non-specific binding. Furthermore, the effect of ligand concentration on adsorption and the kinetics of ligand adsorption to PVC plates were also investigated. Ligand adsorption is a linear function of input up to a limit (around 8.70 ng/mm2) where saturation is reached. The rate of adsorption of pure human IgG to PVC plates was proportionally increased with the temperature, as shown by proportional rate constants almost 2 times faster at 37-degrees-C than at 4-degrees-C. These results have practical implications for investigators using PVC for immunoassays and should be taken into consideration when designing such assays.
引用
收藏
页码:1 / 7
页数:7
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