SEROLOGICAL DETECTION OF ONION YELLOW DWARF VIRUS IN GARLIC

被引：17
|
作者：
KOCH, M [1 ]
SALOMON, R [1 ]
机构：
[1] AGR RES ORG,DEPT VIROL,IL-50250 BET DAGAN,ISRAEL
来源：
PLANT DISEASE | 1994年 / 78卷 / 08期
关键词：
D O I：
10.1094/PD-78-0785
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
A highly sensitive antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) was developed to test for potyvirus in garlic leaves. Antiserum adsorbed with an equal volume of sap from healthy leaves is routinely used at 1:10,000 dilution and is able to detect virus at dilutions up to 1:40,000. Leaf sap can be diluted up to 1:512 when using antibody at the routine concentration. Virus was detected in plants 2 wk after inoculation via aphids. Immunosorbent electron microscopy showed that the potyvirus being tested was only onion yellow dwarf virus (OYDV). All field-grown plants tested were OYDV-infected.
引用
收藏
页码：785 / 788
页数：4
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