HUMAN PANCREATIC PROCOLIPASE EXPRESSED IN INSECT CELLS - PURIFICATION AND CHARACTERIZATION

被引：7
作者：
LOWE, ME
机构：
[1] Department of Pediatrics, Washington University School of Medicine, St Louis
来源：
PROTEIN EXPRESSION AND PURIFICATION | 1994年 / 5卷 / 06期
关键词：
D O I：
10.1006/prep.1994.1080
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA clone encoding human pancreatic procolipase was incorporated into a recombinant baculovirus. Spodoptera frugiperda insect cells infected with the recombinant baculovirus secreted procolipase into the medium, which could be isolated in a single step by immunoaffinity chromatography. The highly purified protein reactivated human pancreatic lipase in a concentration-dependent fashion and was efficiently converted to colipase by limited trypsin digestion. This expression system is suitable for producing amounts of procolipase adequate for biophysical studies. (C) 1994 Academic Press, Inc.
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页码：583 / 586
页数：4
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