DETECTION OF SICKLE-CELL-ANEMIA MUTATION BY COLOR DNA AMPLIFICATION

被引：39
作者：
CHEHAB, FF
KAN, YW
机构：
[1] APPL BIOSYST INC, FOSTER CITY, CA USA
[2] UNIV CALIF SAN FRANCISCO, DEPT MED, SAN FRANCISCO, CA 94143 USA
[3] UNIV CALIF SAN FRANCISCO, DEPT LAB MED, SAN FRANCISCO, CA 94143 USA
[4] UNIV CALIF SAN FRANCISCO, HOWARD HUGHES MED INST, SAN FRANCISCO, CA 94143 USA
来源：
LANCET | 1990年 / 335卷 / 8680期
关键词：
D O I：
10.1016/0140-6736(90)90138-U
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A fluorescence assay to detect the mutation in sickle cell anaemia has been developed. Oligonucleotides homologous to the normal or the mutant DNA sequence are labelled with fluorescein and rhodamine, respectively, and used to prime the polymerase chain reaction. Amplified DNA products with the normal sequence fluoresce green, whereas those with the mutant sequence fluoresce red; heterozygous (AS) DNA fluoresces yellow. The results can be read by eye and the procedure could be automated. © 1990.
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页码：15 / 17
页数：3
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