OXIDATION REDUCTION SENSITIVE INTERACTION OF A CELLULAR 50-KDA PROTEIN WITH AN RNA HAIRPIN IN THE 5' NONCODING REGION OF THE POLIOVIRUS GENOME

被引：67
作者：
NAJITA, L
SARNOW, P
机构：
[1] UNIV COLORADO, HLTH SCI CTR,DEPT BIOCHEM BIOPHYS & GENET,B121, 4200 E 9TH AVE, DENVER, CO 80262 USA
[2] UNIV COLORADO, HLTH SCI CTR, DEPT MICROBIOL & IMMUNOL, DENVER, CO 80262 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 15期
关键词：
α-sarcin; diamide; RNA footprinting; RNA gel retention assay;
D O I：
10.1073/pnas.87.15.5846
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genetic and biochemical analyses of the 5' noncoding region of poliovirus have indicated the importance of this region in both translation and amplification of the viral RNA. The role of the cellular machinery required for these events is just beginning to be revealed. Using an RNA gel retention assay, we have identified a cellular 50-kDa protein that forms a specific complex with a stable stem-loop structure present in the viral 5' noncoding region. The formation of the RNA-protein complex is dependent on the availability of free sulfhydryl groups in the protein. The possible involvement of this RNA-protein complex in the regulation of viral gene expression is discussed.
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页码：5846 / 5850
页数：5
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