EFFICIENT ESTIMATION OF CELL-VOLUME AND NUMBER USING THE NUCLEATOR AND THE DISECTOR

被引：101

作者：

MOLLER, A ^{[1
]}

STRANGE, P ^{[1
]}

GUNDERSEN, HJG ^{[1
]}

机构：

[1] AARHUS UNIV,STEREOL RES LAB,DK-8000 AARHUS,DENMARK

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1990年 / 159卷

关键词：

Disector; neuron sizes; neurons; neuroscience; nuclear size; nuclear‐cytoplasmic ratio; nucleator; particle sizes; particles; point‐sampled intercepts; stereology; volume;

D O I：

10.1111/j.1365-2818.1990.tb03019.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The nucleator allows the unbiased estimation of absolute structural quantities of suitably sampled, arbitrarily shaped structures from observations made from arbitrary points using isotropic probes. A number of time‐saving modifications using the nucleator and the consequences of the modifications are studied in terms of their bias and efficiency. Using rat neocortex as an example, a description is given of how to estimate mean neuronal volume and total neuron number efficiently from only a few pairs of plastic sections with a thickness of about 3 μm. 1990 Blackwell Science Ltd

引用

页码：61 / 71

页数：11

共 16 条

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