PHARMACOLOGICAL CHARACTERIZATION OF MUSCARINIC CHOLINERGIC RECEPTORS IN CAT PONS AND CORTEX - PRELIMINARY-STUDY

Muscarinic receptors (mAChRs) in the pontine reticular formation comprise a critical part of the REM sleep-generating system. Although the role of specific mAChR subtypes remains unclear, data from in vivo microinjection studies suggest that in the pens the M2 subtype is important for REM sleep generation. The present study tested the hypothesis that M2 antagonists would show a greater binding potency in feline pens than M1 or M3 antagonists. Competition binding assays showed 4-DAMP to be more potent than pirenzepine or AF-DX 116 in its ability to displace tritiated quinuclidinyl benzilate, and linear regression analyses indicated that 4-DAMP and pirenzepine each interacted with more than one binding site. These data demonstrate the presence of a mixture of mAChR subtypes in the feline pons, and are consistent with the view that REM sleep is mediated by more than one mAChR subtype.