TIME-BASE MODULATION FOR THE CORRECTION OF CYCLOTRON FREQUENCY-SHIFTS OBSERVED IN LONG-LIVED TRANSIENTS FROM FOURIER-TRANSFORM ION-CYCLOTRON-RESONANCE MASS-SPECTROMETRY OF ELECTROSPRAYED BIOPOLYMERS

被引：45

作者：

BRUCE, JE ^{[1
]}

ANDERSON, GA ^{[1
]}

HOFSTADLER, SA ^{[1
]}

WINGER, BE ^{[1
]}

SMITH, RD ^{[1
]}

机构：

[1] PACIFIC NW LAB, DEPT CHEM SCI, CHEM METHODS & SEPARAT GRP, RICHLAND, WA 99352 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 08期

关键词：

D O I：

10.1002/rcm.1290070803

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The occurrence of small shifts in the cyclotron frequency during the acquisition of very long transients (in excess of 80 s) has been observed to be a limiting factor for ultrahigh-resolution mass measurements of protein ions performed with electrospray-ionization Fourier-transform ion-cyclotron-resonance mass spectrometry. Resolution measurements were restricted to values less than 10(6) because of the frequency shifts. Measurements of the frequency shifts, performed by sequentially transforming small segments of the transient, allowed the shift to be characterized and fitted to a 4th-order equation. The sampling rate of the acquired transient was then modulated (at a rate equal to the reciprocal of the rate for the frequency shift) to allow ultrahigh resolution, greater than 2 X 10(6), and improved mass measurement and precision to be achieved for a small protein.

引用

页码：700 / 703

页数：4

共 24 条

[1] IMPROVED FOURIER-TRANSFORM ION-CYCLOTRON-RESONANCE MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

BEU, SC ;

SENKO, MW ;

QUINN, JP ;

MCLAFFERTY, FW .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (02) :190-192

[2]

BEU SC, IN PRESS J AM SOC MA

[3] AN ION-STORAGE TIME-OF-FLIGHT MASS-SPECTROMETER FOR ANALYSIS OF ELECTROSPRAY IONS [J].

BOYLE, JG ;

WHITEHOUSE, CM ;

FENN, JB .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1991, 5 (09) :400-405

[4]

BOYLE JG, 1992, 40TH P ASMS C MASS S, P1665

[5]

BRUCE JM, UNPUB

[6] MATRIX-ASSISTED LASER DESORPTION IONIZATION OF HIGH-MASS MOLECULES BY FOURIER-TRANSFORM MASS-SPECTROMETRY [J].

CASTRO, JA ;

KOSTER, C ;

WILKINS, C .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (04) :239-241

[7]

Covey T R, 1988, Rapid Commun Mass Spectrom, V2, P249, DOI 10.1002/rcm.1290021111

[8] ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].

FENN, JB ;

MANN, M ;

MENG, CK ;

WONG, SF ;

WHITEHOUSE, CM .

SCIENCE, 1989, 246 (4926) :64-71

[9] DISENTANGLING ELECTROSPRAY SPECTRA WITH MAXIMUM-ENTROPY [J].

FERRIGE, AG ;

SEDDON, MJ ;

GREEN, BN ;

JARVIS, SA ;

SKILLING, J .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (11) :707-711

[10]

HENRY KD, 1989, P NATL ACAD SCI USA, V86, P975

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