THE INEFFECTUALITY OF POTATO PROTEASE INHIBITOR ON THE EXTRACELLULAR PROTEASE FROM ERWINIA-CAROTOVORA SUBSP CAROTOVORA

被引：5
|
作者：
HEILBRONN, J [1 ]
LYON, GD [1 ]
机构：
[1] SCOTTISH CROP RES INST,DUNDEE DD2 5DA,SCOTLAND
来源：
JOURNAL OF APPLIED BACTERIOLOGY | 1990年 / 69卷 / 01期
关键词：
D O I：
10.1111/j.1365-2672.1990.tb02907.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Growth conditions are described for optimum production of extracellular protease in batch cultures of the soft rot pathogen Erwinia carotovora subsp. carotovora. This protease was inhibited by approximately 96% by 1 mmol/1 EDTA and by 55–6% by 10 mmol/l cysteine thereby classifying it as a metalloprotease. It was not inhibited by a chymotrypsin inhibitor extracted from potato tubers. This evidence suggests that the potato chymotrypsin inhibitor is not associated with resistance of potatoes to E. carotovora subsp. carotovora. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码：25 / 29
页数：5
相关论文
共 50 条
  • [21] NONSENSE-SUPPRESSOR MUTANTS OF ERWINIA-CAROTOVORA SUBSP CAROTOVORA
    HINTON, JCD
    PEROMBELON, MCM
    SALMOND, GPC
    FEMS MICROBIOLOGY LETTERS, 1985, 28 (01) : 103 - 106
  • [22] LUMINESCENCE BASED DETECTION OF ERWINIA-CAROTOVORA SUBSP CAROTOVORA IN SOIL
    GRANT, FA
    PROSSER, JI
    KILLHAM, K
    GLOVER, LA
    SOIL BIOLOGY & BIOCHEMISTRY, 1992, 24 (10) : 961 - 967
  • [23] AFFINITY-CHROMATOGRAPHY OF EXOPOLYGALACTURONATE LYASE FROM ERWINIA-CAROTOVORA SUBSP CAROTOVORA
    KEGOYA, Y
    SETOGUCHI, M
    YOKOHIKI, K
    HATANAKA, C
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (04): : 1055 - 1060
  • [24] SEROLOGICAL AND BIOCHEMICAL VARIATION AMONG POTATO STRAINS OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA AND THEIR TAXONOMIC RELATIONSHIP TO OTHER ERWINIA-CAROTOVORA STRAINS
    DEBOER, SH
    VERDONCK, L
    VRUGGINK, H
    HARJU, P
    BANG, HO
    DELEY, J
    JOURNAL OF APPLIED BACTERIOLOGY, 1987, 63 (06): : 487 - 495
  • [25] Isolation of an extracellular protease gene of Erwinia carotovora subsp carotovora strain SCC3193 by transposon mutagenesis and the role of protease in phytopathogenicity
    Marits, R
    Koiv, V
    Laasik, E
    Mäe, A
    MICROBIOLOGY-SGM, 1999, 145 : 1959 - 1966
  • [26] GENETIC TRANSFER FROM ERWINIA-CAROTOVORA SUBSP CAROTOVORA TO ESCHERICHIA-COLI
    WARD, LJ
    COPEMAN, RJ
    PHYTOPATHOLOGY, 1985, 75 (11) : 1320 - 1320
  • [27] MONOCLONAL-ANTIBODIES TO PECTATE LYASE FROM ERWINIA-CAROTOVORA SUBSP CAROTOVORA
    WARD, LJ
    DEBOER, SH
    PHYTOPATHOLOGY, 1987, 77 (12) : 1753 - 1753
  • [28] POTATO EARLY DYING DISEASE IN THE PACIFIC NORTHWEST CAUSED BY ERWINIA-CAROTOVORA PV CAROTOVORA AND ERWINIA-CAROTOVORA PV ATROSEPTICA
    POWELSON, ML
    AMERICAN POTATO JOURNAL, 1985, 62 (04): : 173 - 176
  • [29] STUDIES ON INC-P PLASMIDS IN ERWINIA-CAROTOVORA SUBSP CAROTOVORA
    JAYASWAL, RK
    BRESSAN, RA
    CHARLES, DJ
    HANDA, AK
    FEMS MICROBIOLOGY LETTERS, 1986, 35 (2-3) : 307 - 312
  • [30] EFFECTS OF CONTAMINATION BY ERWINIA-CAROTOVORA SUBSP CAROTOVORA AND ERWINIA-CAROTOVORA SUBSP ATROSEPTICA OF POTATO SEED TUBERS AND OF CULTIVAR RESISTANCE ON BLANKING OR NON-EMERGENCE AND BLACKLEG DEVELOPMENT IN VALENCIA, SPAIN
    PEROMBELON, MCM
    LOPEZ, MM
    CARBONELL, J
    HYMAN, LJ
    POTATO RESEARCH, 1988, 31 (04) : 591 - 599
12345