Ykt6 mediates autophagosome-vacuole fusion

被引：11

作者：

Bas, Levent ^{[1
]}

Papinski, Daniel ^{[1
]}

Kraft, Claudine ^{[1
,2
]}

机构：

[1] Univ Vienna, Vienna Bioctr, Max F Perutz Labs, Vienna, Austria

[2] Univ Freiburg, Fac Med, ZBMZ, Inst Biochem & Mol Biol, Freiburg, Germany

来源：

MOLECULAR & CELLULAR ONCOLOGY | 2018年 / 5卷 / 06期

基金：

欧盟地平线“2020”; 奥地利科学基金会;

关键词：

Autophagy; SNARE; fusion; Ykt6;

D O I：

10.1080/23723556.2018.1526006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Studying the mechanism of autophagosome-vacuole fusion has proven difficult in live yeast cells. Developing a novel in vitro fusion assay, we identified Ykt6 as the missing R-SNARE (Soluble N-ethylmaleimide sensitive factor attachment protein receptor) in this process and pinpoint the place of action of all four SNAREs involved. Parallel studies have confirmed our findings in other organisms.

引用

页数：3

共 8 条

[1] Reconstitution reveals Ykt6 as the autophagosomal SNARE in autophagosome-vacuole fusion [J].

Bas, Levent ;

Papinski, Daniel ;

Licheva, Mariya ;

Torggler, Raffaela ;

Rohringer, Sabrina ;

Schuschnig, Martina ;

Kraft, Claudine .

JOURNAL OF CELL BIOLOGY, 2018, 217 (10) :3656-3669

[2] A novel in vitro assay reveals SNARE topology and the role of Ykt6 in autophagosome fusion with vacuoles [J].

Gao, Jieqiong ;

Reggiori, Fulvio ;

Ungermann, Christian .

JOURNAL OF CELL BIOLOGY, 2018, 217 (10) :3670-3682

[3] The Ccz1-Mon1-Rab7 module and Rab5 control distinct steps of autophagy [J].

Hegedus, Krisztina ;

Takats, Szabolcs ;

Boda, Attila ;

Jipa, Andras ;

Nagy, Peter ;

Varga, Kata ;

Kovacs, Attila L. ;

Juhasz, Gabor .

MOLECULAR BIOLOGY OF THE CELL, 2016, 27 (20) :3132-3142

[4] The Hairpin-type Tail-Anchored SNARE Syntaxin 17 Targets to Autophagosomes for Fusion with Endosomes/Lysosomes [J].

Itakura, Eisuke ;

Kishi-Itakura, Chieko ;

Mizushima, Noboru .

CELL, 2012, 151 (06) :1256-1269

[5] Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17 [J].

Matsui, Takahide ;

Jiang, Peidu ;

Nakano, Saori ;

Sakamaki, Yuriko ;

Yamamoto, Hayashi ;

Mizushima, Noboru .

JOURNAL OF CELL BIOLOGY, 2018, 217 (08) :2633-2645

[6] Autophagosome Maturation and Fusion [J].

Reggiori, Fulvio ;

Ungermann, Christian .

JOURNAL OF MOLECULAR BIOLOGY, 2017, 429 (04) :486-496

[7] Non-canonical role of the SNARE protein Ykt6 in autophagosome-lysosome fusion [J].

Takats, Szabolcs ;

Glatz, Gabor ;

Szenci, Gyozo ;

Boda, Attila ;

Horvath, Gabor V. ;

Hegedus, Krisztina ;

Kovacs, Attila L. ;

Juhasz, Gabor .

PLOS GENETICS, 2018, 14 (04)

[8] Autophagosomal Syntaxin 17-dependent lysosomal degradation maintains neuronal function in Drosophila [J].

Takats, Szabolcs ;

Nagy, Peter ;

Varga, Agnes ;

Pircs, Karolina ;

Karpati, Manuela ;

Varga, Kata ;

Kovacs, Attila L. ;

Hegedus, Krisztina ;

Juhasz, Gabor .

JOURNAL OF CELL BIOLOGY, 2013, 201 (04) :531-539

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