MULTIPLE VIBRIO-VULNIFICUS STRAINS IN OYSTERS AS DEMONSTRATED BY CLAMPED HOMOGENEOUS ELECTRIC-FIELD GEL-ELECTROPHORESIS

被引：54
作者：
BUCHRIESER, C
GANGAR, VV
MURPHREE, RL
TAMPLIN, ML
KASPAR, CW
机构：
[1] UNIV WISCONSIN,FOOD RES INST,MADISON,WI 53706
[2] UNIV FLORIDA,INST FOOD & AGR SCI,DEPT HOME ECONOM,GAINESVILLE,FL 32611
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 03期
关键词：
D O I：
10.1128/AEM.61.3.1163-1168.1995
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Clamped homogeneous electric field gel electrophoresis and a computer program for managing electrophoresis handing patterns (ELBAMAP) were used to analyze genomic DNA of 118 Vibrio vulnificus strains, isolated from three oysters by direct plating. Analysis with SfiI resulted in 60 restriction endonuclease digestion profiles (REDP), while analysis with SrfI produced 53 different REDP. Similarities between REDP ranged from 7 to 93%. Principal-component analysis showed that the strains were heterogeneous.
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页码：1163 / 1168
页数：6
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