A ZYMOGRAM METHOD TO DETECT ENDOGLUCANASES FROM BACILLUS-SUBTILIS, MYROTHECIUM-VERRUCARIA AND TRICHODERMA-REESEI

被引：11

作者：

HOLT, SM ^{[1
]}

HARTMAN, PA ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT MIROBIOL IMMUNOL & PREVENT MED,205 SCI 1,AMES,IA 50011

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1994年 / 13卷 / 01期

关键词：

BACILLUS-SUBTILIS; MYROTHECIUM-VERRUCARIA; TRICHODERMA-REESEI; ENDOGLUCANASE;

D O I：

10.1007/BF01569654

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A purified endoglucanase (Eg) from Bacillus subtilis and crude Eg preparations from Myrothecium verrucaria and Trichoderma reesei were denatured by heat in the presence of SDS and separated by using SDS polyacrylamide gel electrophoresis. Eg zymogram activity bands were detected directly in carboxymethylcellulose-laden separating gels after enzyme renaturation. Denaturing zymogram analysis was a simple method for the separation and detection of Egs.

引用

页码：2 / 4

页数：3

共 10 条

[1] DOMINGUEZ JM, 1992, BIOTECHNOL APPL BIOC, V15, P236
[2] ENZYMATIC-HYDROLYSIS OF CELLULOSE - IS THE CURRENT THEORY OF THE MECHANISMS OF HYDROLYSIS VALID
ENARI, TM
NIKUPAAVOLA, ML
[J]. CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, 1987, 5 (01): : 67 - 87
[3] MINIATURIZATION OF 3 CARBOHYDRATE ANALYSES USING A MICROSAMPLE PLATE READER
FOX, JD
ROBYT, JF
[J]. ANALYTICAL BIOCHEMISTRY, 1991, 195 (01) : 93 - 96
[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4
LAEMMLI, UK
[J]. NATURE, 1970, 227 (5259) : 680 - +
[5] CHARACTERIZATION OF A CELLULOSE-BINDING, CELLULASE-CONTAINING COMPLEX IN CLOSTRIDIUM-THERMOCELLUM
LAMED, R
SETTER, E
BAYER, EA
[J]. JOURNAL OF BACTERIOLOGY, 1983, 156 (02) : 828 - 836
[6] MANDELS M, 1978, PROCESS BIOCHEM, V13, P6
[7] DIFFERENTIAL REGULATION OF SYNTHESIS OF MULTIPLE FORMS OF SPECIFIC ENDOGLUCANASES BY TRICHODERMA-REESEI QM9414
MESSNER, R
GRUBER, F
KUBICEK, CP
[J]. JOURNAL OF BACTERIOLOGY, 1988, 170 (08) : 3689 - 3693
[8] ACTIVITY STAINING OF CELLULASES IN POLYACRYLAMIDE GELS CONTAINING MIXED LINKAGE BETA-GLUCANS
SCHWARZ, WH
BRONNENMEIER, K
GRABNITZ, F
STAUDENBAUER, WL
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 164 (01) : 72 - 77
[9] SPREY B, 1983, FEMS MICROBIOL LETT, V18, P217
[10] WHITAKER DR, 1952, ARCH BIOCHEM BIOPHYS, V43, P253

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