EXTRACELLULAR MANNANASES AND GALACTANASES FROM SELECTED FUNGI

被引：30

作者：

ARAUJO, A ^{[1
]}

WARD, OP ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1990年 / 6卷 / 03期

关键词：

Galactanase; Mannanase; Mannosidase; Talaromyces; Thermoascus; Thielavia;

D O I：

10.1007/BF01577692

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Eight thermophilic fungi were tested for production of mannanases and galactanases. Highest mannanase activities were produced by Talaromyces byssochlamydoides and Talaromyces emersonii. Mannanases from all strains tested were induced by locust bean gum except in the case of Thermoascus aurantiacus, where mannose had a greater inducing effect. Locust bean gum was also the best inducer of β-mannosidase and galactanase except in the case of T. emersonii where galactose was a better inducer of both these enzymes. Highest mannanase activity was produced by Talaromyces species when peptone was used as nitrogen source whereas sodium nitrate promoted maximum production of this enzyme by Thielavia terrestris and T. aurantiacus. The pH optima of mannanases from the thermophilic fungi were in the range 5.0-6.6 and contrasted with the low pH optimum (3.2) of the enzyme from Aspergillus niger. Galactanases had pH optima in the range 4.3-5.8. The mannanase from T. emersonii and the galactanase from T. terrestris were most thermostable, each retaining 100% activity for 3 h at 60°C. © 1990 Society for Industrial Microbiology.

引用

页码：171 / 178

页数：8

共 42 条

[1] Araki T., Kitamikado M., β-Mannanase of bacteria isolated from natural habitats, NIPPON SUISAN GAKKAISHI, 47, pp. 753-760, (1981)
[2] Araujo A., D'Souza J., Production of biomass from enzymatic hydrolysate of agricultural waste, J. Ferment. Technol., 58, pp. 399-401, (1980)
[3] Araujo A., Ward O.P., Hemicellulases of Bacillus species: Preliminary comparative studies on production and properties of mannanases and galactanases, J. Appl. Bacteriol., 68, pp. 253-261, (1990)
[4] Bailey P.J., Liese W., Roesch R., Keilich G., Afting E.G., Cellulase (β-1,4-glucan 4-glucanohydrolase) from the wood=degrading fungus, Polyporus schweinitzii. Fr, Biochim. Biophys. Acta, 185, pp. 381-391, (1969)
[5] Bouquelet S., Spik G., Montreuil J., Properties of a β- d-mannosidase of Aspergillus niger, Biochim. Biophys. Acta, 522, pp. 521-530, (1978)
[6] Brock T., Introduction, an Overview of the Thermophiles, General Molecular and Applied Microbiology, pp. 1-16, (1986)
[7] Civas A., Eberhard R., Le Dizet P., Petek F., Glucosidases induced in Aspergillus tamarii: α- d-galactosidase and β- d-mannanase, Biochem. J., 219, 3, (1984)
[8] Coughlan M.P., The properties of fungal and bacterial cellulases with comment on their production and application, Biotechnology and Genetic Engineering Reviews Vol. 3, pp. 39-109, (1985)
[9] Dekker R.F.H., Biodegradation of the hemicelluloses, Biosynthesis and Biodegradation of Wood components, pp. 505-533, (1985)
[10] Dekker R.F.H., Richards G.N., Hemicellulases: their occurrence, purification, properties and mode of action, Adv. Carbohyd. Chem., 32, pp. 277-352, (1976)

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