CONSTRUCTION OF A PANEL OF CHROMOSOME-SPECIFIC OLIGONUCLEOTIDE PROBES (PRINS-PRIMERS) USEFUL FOR THE IDENTIFICATION OF INDIVIDUAL HUMAN-CHROMOSOMES IN-SITU

被引：66

作者：

KOCH, J ^{[1
]}

HINDKJAER, J ^{[1
]}

KOLVRAA, S ^{[1
]}

BOLUND, L ^{[1
]}

机构：

[1] AARHUS UNIV,INST HUMAN GENET,DK-8000 AARHUS,DENMARK

来源：

CYTOGENETICS AND CELL GENETICS | 1995年 / 71卷 / 02期

关键词：

D O I：

10.1159/000134094

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We present the sequences of a set of oligonucleotides that, when used as primers for PRimed IN Situ (PRINS) labeling, are diagnostic for repetitive sequences in specific human chromosomes. Combined, they enable identification of all human chromosomes except 6, 19, and 20. However, as is also the case with cloned centromeric hybridization probes, chromosomes 14 and 22 are stained together. Along with the sequences of these oligonucleotides we offer a simple, universal procedure for their use. We also present an oligonucleotide that binds to both strands of alpha-satellite DNA, making it possible to specifically amplify alpha-satellite DNA by PCR with this one oligonucleotide alone as primer. The origin of the alpha-satellite DNA in the starting material (whether somatic cell hybrids, flow-sorted chromosomes, or microdissected material) can then be determined on test metaphase spreads by in situ hybridization or PRINS with the PCR product.

引用

页码：142 / 147

页数：6

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