IDENTIFICATION OF MUTATIONS IN 23S RIBOSOMAL-RNA GENE OF CLARITHROMYCIN-RESISTANT MYCOBACTERIUM-INTRACELLULARE

被引：158

作者：

MEIER, A

KIRSCHNER, P

SPRINGER, B

STEINGRUBE, VA

BROWN, BA

WALLACE, RJ

BOTTGER, EC

机构：

[1] HANNOVER MED SCH,INST MED MIKROBIOL,D-30623 HANNOVER,GERMANY

[2] UNIV TEXAS,CTR HLTH,DEPT MICROBIOL,TYLER,TX 75710

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1994年 / 38卷 / 02期

关键词：

D O I：

10.1128/AAC.38.2.381

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clarithromycin is a potent macrolide that has been used for treating infections with nontuberculous mycobacteria. Pairs of susceptible and resistant Mycobacterium intracellulare strains were obtained from patients with chronic pulmonary M. intracellulare infections undergoing monotherapy with clarithromycin. Nucleotide sequence comparisons of the peptidyltransferase region in 23S rRNAs from parental and resistant strains revealed that in three of six resistant strains, for which the MIC was > 32 mu g/ml, a single base was mutated (Escherichia coli equivalent, A-2058 --> G, C, or U). As the modification of adenine 2058 by dimethylation is a frequent cause of macrolide resistance in a variety of different bacteria, we suggest that mutation of A-2058 confers acquired resistance to clarithromycin in M. intracellulare.

引用

收藏

页码：381 / 384

页数：4

相关论文

共 31 条

[1] ACTIVITIES OF CLARITHROMYCIN AGAINST 8 SLOWLY GROWING SPECIES OF NONTUBERCULOUS MYCOBACTERIA, DETERMINED BY USING A BROTH MICRODILUTION MIC SYSTEM [J].

BROWN, BA ;

WALLACE, RJ ;

ONYI, GO .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (09) :1987-1990

[2] ACTIVITIES OF 4 MACROLIDES, INCLUDING CLARITHROMYCIN, AGAINST MYCOBACTERIUM-FORTUITUM, MYCOBACTERIUM-CHELONAE, AND M-CHELONAE-LIKE ORGANISMS [J].

BROWN, BA ;

WALLACE, RJ ;

ONYI, GO ;

DEROSAS, V ;

WALLACE, RJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (01) :180-184

[3] ACTIVITY OF CLARITHROMYCIN AGAINST MYCOBACTERIUM-AVIUM INFECTION IN PATIENTS WITH ACQUIRED-IMMUNE-DEFICIENCY-SYNDROME - A CONTROLLED CLINICAL-TRIAL [J].

DAUTZENBERG, B ;

TRUFFOT, C ;

LEGRIS, S ;

MEYOHAS, MC ;

BERLIE, HC ;

MERCAT, A ;

CHEVRET, S ;

GROSSET, J .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (03) :564-569

[4] CHLORAMPHENICOL-ERYTHROMYCIN RESISTANCE MUTATIONS IN A 23S RIBOSOMAL-RNA GENE OF ESCHERICHIA-COLI [J].

ETTAYEBI, M ;

PRASAD, SM ;

MORGAN, EA .

JOURNAL OF BACTERIOLOGY, 1985, 162 (02) :551-557

[5] MOLECULAR-BASIS OF STREPTOMYCIN RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS - ALTERATIONS OF THE RIBOSOMAL-PROTEIN S12 GENE AND POINT MUTATIONS WITHIN A FUNCTIONAL 16S RIBOSOMAL-RNA PSEUDOKNOT [J].

FINKEN, M ;

KIRSCHNER, P ;

MEIER, A ;

WREDE, A ;

BOTTGER, EC .

MOLECULAR MICROBIOLOGY, 1993, 9 (06) :1239-1246

[6]

Gale EF, 1981, MOL BASIS ANTIBIOTIC

[7] MAPPING OF CHLOROPLAST MUTATIONS CONFERRING RESISTANCE TO ANTIBIOTICS IN CHLAMYDOMONAS - EVIDENCE FOR A NOVEL SITE OF STREPTOMYCIN RESISTANCE IN THE SMALL SUBUNIT RIBOSOMAL-RNA [J].

GAUTHIER, A ;

TURMEL, M ;

LEMIEUX, C .

MOLECULAR & GENERAL GENETICS, 1988, 214 (02) :192-197

[8]

HARRIS EH, 1989, GENETICS, V123, P281

[9] CLARITHROMYCIN MINIMAL INHIBITORY AND BACTERICIDAL CONCENTRATIONS AGAINST MYCOBACTERIUM-AVIUM [J].

HEIFETS, LB ;

LINDHOLMLEVY, PJ ;

COMSTOCK, RD .

AMERICAN REVIEW OF RESPIRATORY DISEASE, 1992, 145 (04) :856-858

[10] SELECTION OF RESISTANT MUTANTS OF MYCOBACTERIUM-AVIUM IN BEIGE MICE BY CLARITHROMYCIN MONOTHERAPY [J].

JI, BH ;

LOUNIS, N ;

TRUFFOTPERNOT, C ;

GROSSET, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (12) :2839-2840

← 1 2 3 4 →