ISOLATION AND CHARACTERIZATION OF GENES ENCODING CHAPERONIN 60-BETA FROM ARABIDOPSIS-THALIANA

被引:27
|
作者
ZABALETA, E
OROPEZA, A
JIMENEZ, B
SALERNO, G
CRESPI, M
HERRERAESTRELLA, L
机构
[1] FIBA, CTR INVEST BIOL, MAR DEL PLATA, ARGENTINA
[2] CINVESTAV IPN, DEPT INGN GENET PLANTAS, IRAPUATO, GUANAJUATO, MEXICO
关键词
RECOMBINANT DNA; GENOMIC LIBRARY; PHAGE-LAMBDA VECTOR; PROMOTER SEQUENCE; SUGAR INDUCTION; WHEAT; EXON; INTRON;
D O I
10.1016/0378-1119(92)90685-I
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Chaperonins (Cpn) are implicated in the folding and assembly of multimeric proteins in plastids and mitochondria of eukaryotes and in prokaryotes. Plastid Cpn is composed of two different polypeptides termed Cpn60-alpha and Cpn60-beta. We have isolated cDNA and genomic clones encoding Cpn60-beta from Arabidopsis thaliana. The steady-state level of the cpn60-beta mRNAs is higher in etiolated leaves and sucrose-treated plants as compared to control leaves. The A. thaliana cpn60-beta gene family consists of at least three different coding units. It was confirmed that Cpn-beta-encoding genes have a high level of conservation among plants.
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页码:175 / 181
页数:7
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