TRANSFECTION OF CALCITONIN GENE REGULATORY ELEMENTS INTO A CELL-CULTURE MODEL OF THE C-CELL

被引：18
作者：
COTE, GJ
ABRUZZESE, RV
LIPS, CJM
GAGEL, RF
机构：
[1] VET ADM MED CTR, DEPT MED,MOLEC & CELLULAR ENDOCRINOL LAB,111E, 2002 HOLCOMBE BLVD, HOUSTON, TX 77030 USA
[2] VET ADM MED CTR, DEPT CELL BIOL, MOLEC & CELLULAR ENDOCRINOL LAB, HOUSTON, TX 77211 USA
[3] STATE UNIV UTRECHT HOSP, DEPT INTERNAL MED, 3511 GV UTRECHT, NETHERLANDS
来源：
JOURNAL OF BONE AND MINERAL RESEARCH | 1990年 / 5卷 / 02期
关键词：
D O I：
10.1002/jbmr.5650050210
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Calcitonin gene expression in the TT cell line can be regulated by phorbol esters, cAMP, glucocorticoids, and 1,25‐dihydroxyvitamin D3. To further study the regulation of this gene we have sequenced 1460 bases 5′ to the start of calcitonin gene transcription. This DNA sequence contains cis consensus elements for both phorbol ester‐ and cAMP‐responsive elements. To study the role of these elements, calcitonin 5′ flanking DNA was coupled to the human growth hormone gene as a reporter and transiently transfected into TT cells, a human thyroid C cell line. Treatment of transfected TT cells stimulated a two‐ to fivefold increase in reported gene product expression, confirming the existence of functional cAMP‐ and phorbol ester‐dependent enhancers within the calcitonin 5′ flanking sequence Copyright © 1990 ASBMR
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页码：165 / 171
页数：7
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