Physiological restriction of the L-lactic acid production by Rhizopus arrhizus

被引:16
|
作者
Rosenberg, M
Kristofikova, L
机构
[1] Slovak Technical University, Department of Biotechemical, Technology Radlinského, Bratislava
来源
ACTA BIOTECHNOLOGICA | 1995年 / 15卷 / 04期
关键词
D O I
10.1002/abio.370150409
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The influence of basic physiological factors on the quality of inocula and L(+)-lactic acid production by Rhizopus arrhizus CCM 8109 were studied. The most effective preparation of the spores (5x10(7) spores/ml) and subsequent good lactate production was achieved on the agar medium with soil extract and malt agar. The optimum initial amount of active spores for inoculation was 10(3)-10(4) spores/ml. The preparation of inoculum required intensive stirring with lower aeration and pH maintained in the range from 4.8 to 6.0 by the addition of CaCO3. The maximum yield of lactic acid production was achieved by using 5% (v/v) of 24-h-old inoculum. The intensity of lactic acid production in the inoculum was proportional to its production in the subsequent steps of fermentation and can be used as a fast control of the physiological state of the producers.
引用
收藏
页码:367 / 374
页数:8
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