DETERMINATION OF THE PETROSELINIC ACID IN SEEDS OF CORIANDRUM-SATIVUM BY GAS-LIQUID-CHROMATOGRAPHY AS N-BUTYL ESTERS

Oleic acid (18:1 n9), vaccenic acid (18:1 n7), and petroselinic acid (18:1 n12) can not be separated by gas chromatography (GLC) as their methylesters. But separations of their n-butyl esters are feasible. With 0.1 mol Na-tert-butanol as catalyst, dissolved in n-butanol, an extraction from the seed grist and simultaneous transbutylation of the lipids can be achieved within 20 min at room temperature. Using a capillary column (60 m DB-23, 0.25 mu, J & W, No. 122-2362), hydrogen as carrier gas and an oven temperature of 220 degrees C, one analysis can be performed within 13 min.