A RAPID COLORIMETRIC ASSAY OF KILLER TOXIN ACTIVITY IN YEAST

被引：0
|
作者：
HODGSON, VJ
WALKER, GM
BUTTON, D
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1994年 / 120卷 / 1-2期
关键词：
MTT-COLORIMETRIC ASSAY; KILLER TOXIN; WILLIOPSIS MRAKII; CANDICIDAL ACTIVITY;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.
引用
收藏
页码：201 / 205
页数：5
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