A RAPID COLORIMETRIC ASSAY OF KILLER TOXIN ACTIVITY IN YEAST

被引：0
|
作者：
HODGSON, VJ
WALKER, GM
BUTTON, D
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1994年 / 120卷 / 1-2期
关键词：
MTT-COLORIMETRIC ASSAY; KILLER TOXIN; WILLIOPSIS MRAKII; CANDICIDAL ACTIVITY;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is reduced to a dark blue end-product, MTT-Formazan, by the mitochondrial dehydrogenases of living cells. MTT reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of Williopsis mrakii killer toxin against sensitive cells of Candida glabrata. The MTT assay is rapid, quantitative and compares favourably with traditional plating techniques for the assessment of sensitive viability.
引用
收藏
页码：201 / 205
页数：5
相关论文
共 50 条
  • [31] A novel killer toxin produced by the marine-derived yeast Wickerhamomyces anomalus YF07b
    Guo, Feng-Jun
    Ma, Yan
    Xu, Hong-Mei
    Wang, Xiang-Hong
    Chi, Zhen-Ming
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2013, 103 (04): : 737 - 746
  • [32] LINEAR DNA PLASMIDS OF PICHIA-INOSITOVORA ARE ASSOCIATED WITH A NOVEL KILLER TOXIN ACTIVITY
    HAYMAN, GT
    BOLEN, PL
    CURRENT GENETICS, 1991, 19 (05) : 389 - 393
  • [33] KILLER TOXIN OF HANSENIASPORA-UVARUM
    RADLER, F
    SCHMITT, MJ
    MEYER, B
    ARCHIVES OF MICROBIOLOGY, 1990, 154 (02) : 175 - 178
  • [34] Circular dichroism analysis of the interaction between the alpha and beta subunits in a killer toxin produced by a halotolerant yeast, Pichia farinosa
    Suzuki, C
    Kashiwagi, T
    Tsuchiya, F
    Kunishima, N
    Morikawa, K
    Nikkuni, S
    Arata, Y
    PROTEIN ENGINEERING, 1997, 10 (02): : 99 - 101
  • [35] Kluyveromyces wickerhamii killer toxin: purification and activity towards Brettanomyces/Dekkera yeasts in grape must
    Comitini, Francesca
    Ciani, Maurizio
    FEMS MICROBIOLOGY LETTERS, 2011, 316 (01) : 77 - 82
  • [36] Killer toxin of Saccharomyces cerevisiae Y500-4L active against Fleischmann and Itaiquara commercial brands of yeast
    Soares, GAM
    Sato, HH
    REVISTA DE MICROBIOLOGIA, 1999, 30 (03): : 253 - 257
  • [37] Yeast Viral Killer Toxin K1 Induces Specific Host Cell Adaptions via Intrinsic Selection Pressure
    Gier, Stefanie
    Simon, Martin
    Gasparoni, Gilles
    Khalifa, Salem
    Schulz, Marcel H.
    Schmitt, Manfred J.
    Breinig, Frank
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2020, 86 (04)
  • [38] KINETIC-STUDIES OF KILLER TOXIN K1 BINDING TO YEAST-CELLS INDICATE 2 RECEPTOR POPULATIONS
    KURZWEILOVA, H
    SIGLER, K
    ARCHIVES OF MICROBIOLOGY, 1994, 162 (03) : 211 - 214
  • [39] Alanine-scanning mutagenesis of HM-1 killer toxin and the essential residues for killing activity
    Miyamoto, M
    Han, GD
    Kimura, T
    Furuichi, Y
    Komiyama, T
    JOURNAL OF BIOCHEMISTRY, 2005, 137 (04) : 517 - 522
  • [40] Analysis of Yeast Killer Toxin K1 Precursor Processing via Site-Directed Mutagenesis: Implications for Toxicity and Immunity
    Gier, Stefanie
    Schmitt, Manfred J.
    Breinig, Frank
    MSPHERE, 2020, 5 (01):
12345