IDENTIFICATION OF A TRANSCRIPTIONAL ENHANCER ELEMENT UPSTREAM FROM THE PROTO-ONCOGENE FOS

被引：150
作者：
DESCHAMPS, J
MEIJLINK, F
VERMA, IM
机构：
来源：
SCIENCE | 1985年 / 230卷 / 4730期
关键词：
D O I：
10.1126/science.3865371
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Sequences upstream from the proto-ongogene fos were shown to be essential for its transcription. Transient expression of the chloramphenicol acetyltransferase (CAT) gene linked to upstream sequences of the fos gene including its promoter reveals that sequences located 64 to 404 base pairs 5'' to the fos cap site contain a typical transcriptional enhancer. Moreover, these enhancer sequences, which are strikingly conserved between mouse and human fos genes, coincide with a deoxyribonuclease I-hypersensitive site in the chromatin. The expression of the fos-CAT fusion genes was stimulated only two to three times by the fos inducer 12-0-tetradecanoyl phorbol-13-acetate. The fos enhancer does not appear to be tissue-specific.
引用
收藏
页码：1174 / 1177
页数：4
相关论文
共 28 条
[1]   A LYMPHOCYTE-SPECIFIC CELLULAR ENHANCER IS LOCATED DOWNSTREAM OF THE JOINING REGION IN IMMUNOGLOBULIN HEAVY-CHAIN GENES [J].
BANERJI, J ;
OLSON, L ;
SCHAFFNER, W .
CELL, 1983, 33 (03) :729-740
[2]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[3]   3 REGIONS UPSTREAM FROM THE CAP SITE ARE REQUIRED FOR EFFICIENT AND ACCURATE TRANSCRIPTION OF THE RABBIT BETA-GLOBIN GENE IN MOUSE 3T6 CELLS [J].
DIERKS, P ;
VANOOYEN, A ;
COCHRAN, MD ;
DOBKIN, C ;
REISER, J ;
WEISSMANN, C .
CELL, 1983, 32 (03) :695-706
[4]   ALTERNATIVE SETS OF DNASE I-HYPERSENSITIVE SITES CHARACTERIZE THE VARIOUS FUNCTIONAL-STATES OF THE CHICKEN LYSOZYME GENE [J].
FRITTON, HP ;
IGOKEMENES, T ;
NOWOCK, J ;
STRECHJURK, U ;
THEISEN, M ;
SIPPEL, AE .
NATURE, 1984, 311 (5982) :163-165
[5]   A TISSUE-SPECIFIC TRANSCRIPTION ENHANCER ELEMENT IS LOCATED IN THE MAJOR INTRON OF A REARRANGED IMMUNOGLOBULIN HEAVY-CHAIN GENE [J].
GILLIES, SD ;
MORRISON, SL ;
OI, VT ;
TONEGAWA, S .
CELL, 1983, 33 (03) :717-728
[6]   RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].
GORMAN, CM ;
MOFFAT, LF ;
HOWARD, BH .
MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051
[7]   NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].
GRAHAM, FL ;
VANDEREB, AJ .
VIROLOGY, 1973, 52 (02) :456-467
[8]   STIMULATION OF 3T3 CELLS INDUCES TRANSCRIPTION OF THE C-FOS PROTO-ONCOGENE [J].
GREENBERG, ME ;
ZIFF, EB .
NATURE, 1984, 311 (5985) :433-438
[9]   INDUCTION OF ALTERED CHROMATIN STRUCTURES BY SIMIAN VIRUS-40 ENHANCER AND PROMOTER ELEMENTS [J].
JONGSTRA, J ;
REUDELHUBER, TL ;
OUDET, P ;
BENOIST, C ;
CHAE, CB ;
JELTSCH, JM ;
MATHIS, DJ ;
CHAMBON, P .
NATURE, 1984, 307 (5953) :708-714
[10]   ENHANCER ELEMENTS [J].
KHOURY, G ;
GRUSS, P .
CELL, 1983, 33 (02) :313-314
123