BACULOVIRUS EXPRESSED HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEIN-C PROTECTS MICE FROM LETHAL HSV-1 INFECTION

被引：31

作者：

GHIASI, H ^{[1
]}

KAIWAR, R ^{[1
]}

NESBURN, AB ^{[1
]}

WECHSLER, SL ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT OPHTHALMOL,LOS ANGELES,CA 90024

来源：

ANTIVIRAL RESEARCH | 1992年 / 18卷 / 3-4期

关键词：

GLYCOPROTEIN-C; HSV-1; IMMUNOGENICITY; BACULOVIRUS;

D O I：

10.1016/0166-3542(92)90062-A

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A recombinant baculovirus (vAc-gCl) was constructed that expresses the glycoprotein C (gC) gene of herpes simplex virus type 1 (HSV-1). When Sf9 cells were infected with this recombinant, a protein that was smaller in size than authentic HSV-1 gC was detected by Western blotting using anti-gC polyclonal antibody. The recombinant gC was susceptible to tunicamycin, partially resistant to Endo-H, and was found on the membrane of Sf9 cells. Antibodies raised in mice to recombinant gC reacted with gC from HSV-1 infected cells and neutralized the infectivity of HSV-1 in vitro. Immunized mice were protected from lethal challenge with HSV-1.

引用

页码：291 / 302

页数：12