INTERACTION OF MERCURIC IONS WITH THE BACTERIAL-GROWTH MEDIUM AND ITS EFFECTS ON ENZYMATIC REDUCTION OF MERCURY

被引：41

作者：

CHANG, JS

HONG, J

OGUNSEITAN, OA

OLSON, BH

机构：

[1] UNIV CALIF IRVINE,BIOCHEM ENGN PROGRAM,IRVINE,CA 92717

[2] UNIV CALIF IRVINE,DEPT ENVIRONM ANAL & DESIGN,IRVINE,CA 92717

来源：

BIOTECHNOLOGY PROGRESS | 1993年 / 9卷 / 05期

关键词：

D O I：

10.1021/bp00023a012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The interaction of Hg2+ with components of Luria-Bertani broth (LB), used to grow the mercury-resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30-40 % of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell-free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+-LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ Could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).

引用

页码：526 / 532

页数：7

共 36 条

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