ACTIVATION OF PROTHROMBIN IN THE PRESENCE OF HUMAN UMBILICAL-VEIN ENDOTHELIAL-CELLS

被引：12
|
作者：
SCHOEN, P [1 ]
REUTELINGSPERGER, C [1 ]
LINDHOUT, T [1 ]
机构：
[1] UNIV LIMBURG,CARDIOVASC RES INST MAASTRICHT,DEPT BIOCHEM,POB 616,6200 MD MAASTRICHT,NETHERLANDS
来源：
BIOCHEMICAL JOURNAL | 1992年 / 281卷
关键词：
D O I：
10.1042/bj2810661
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Addition of Factor Xa, Factor Va and prothrombin to immobilized cultured human umbilical-vein endothelial cells resulted after a time delay in thrombin formation. The prothrombin-converting (prothrombinase) activity, however, was not associated with the cell surface. Rather, perturbation by thrombin, either formed in situ or exogenously added, induced a procoagulant phospholipid surface in the fluid phase, which, in the presence of Factor Xa and Factor Va, enabled the assembly of prothrombinase.
引用
收藏
页码：661 / 664
页数：4
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