NA+-DEPENDENT HIGH-AFFINITY UPTAKE OF L-GLUTAMATE IS PRIMARY CULTURES OF HUMAN FIBROBLASTS ISOLATED FROM 3 DIFFERENT TYPES OF TISSUE

被引：23

作者：

BALCAR, VJ ^{[1
]}

SHEN, J ^{[1
]}

BAO, SS ^{[1
]}

KING, NJC ^{[1
]}

机构：

[1] UNIV SYDNEY,DEPT PATHOL,SYDNEY,NSW 2006,AUSTRALIA

来源：

FEBS LETTERS | 1994年 / 339卷 / 1-2期

关键词：

L-GLUTAMATE; TRANSPORT; UPTAKE INHIBITOR; FIBROBLAST; HUMAN;

D O I：

10.1016/0014-5793(94)80382-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [H-3]L-glutamate by a Na+-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-thleo-3-hydroxyaspartate and L-trans-pyrrolidine-2,4-dicarboxylate but not D-glutamate. It was also reduced by elevated concentrations of K+, Rb+ and Cs+. The values of K-m's were 5-20 mu M, well within the 'high affinity' region. Variations in the capacity (V-max) of [H-3]L-glutamate uptake did not correlate with the origin (muscle, skin or nerve tissue) of the fibroblasts. The uptake characteristics suggest that it is mediated by a transport system similar to that commonly observed only in brain tissue.

引用

页码：50 / 54

页数：5

共 34 条

[1] STEREOSPECIFICITY OF INHIBITION OF L-GLUTAMATE AND L-ASPARTATE HIGH AFFINITY UPTAKE IN RAT-BRAIN SLICES BY THREO-3-HYDROXYASPARTATE [J].